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目的探讨槲皮素对人胃癌细胞SGC-7901上皮间质转化的影响及机制。方法胃癌细胞SGC-7901分为对照组(普通培养液)、LY294002组(培养液+LY294002)、槲皮素组(培养液+槲皮素);采用MTT法检测槲皮素对胃癌细胞SGC-7901增殖的影响,依据细胞生长抑制率计算槲皮素对胃癌细胞SGC-7901的半数抑制浓度(median inhibitory concentration,IC_(50))值,参照IC_(50)值进行后续实验;采用划痕试验检测3组胃癌细胞SGC-7901增殖、迁移能力;采用Western blot法检测胃癌细胞AKT、p-AKT、Snail、Vimentin及E-钙黏附素(E-cadherin)蛋白表达情况;采用荧光定量PCR法检测3组胃癌细胞上皮间质转化相关因子Snail、Vimentin及E-cadherinmRNA的表达情况。结果槲皮素对胃癌细胞SGC-7901有明显的增殖抑制作用,其IC_(50)为(112.9±2.05)μmol/L;LY294002组胃癌细胞迁移距离[(0.16±0.03)mm]、槲皮素组胃癌细胞迁移距离[(0.15±0.02)mm]均低于对照组[(0.44±0.04)mm](P<0.05),LY294002组与槲皮素组比较差异无统计学意义(P>0.05);Snail、Vimentin、p-AKT蛋白表达水平在LY294002组(0.760±0.003,0.750±0.006,0.71±0.03)、槲皮素组(0.750±0.006,0.690±0.004,0.68±0.02)均明显低于对照组(1,1,1),E-cadherin表达在LY294002组(2.89±0.19)与槲皮素组(3.66±0.18)均高于对照组(1)(P<0.05);LY294002组、槲皮素组、对照组AKT蛋白表达水平(1.03±0.02,1.02±0.01,1)比较差异无统计学意义(P>0.05);Snail、Vimentin mRNA表达水平在LY294002组(0.72±0.02,0.78±0.03)、槲皮素组(0.71±0.01,0.79±0.03)均明显低于对照组(1,1),E-cadherin mRNA表达水平在LY294002组(2.51±0.08)、槲皮素组(2.77±0.16)均高于对照组(1)(P<0.05);LY294002组Snail、Vimentin、E-cadherin mRNA及Snail、Vimentin、E-cadherin、p-AKT蛋白表达水平与槲皮素组比较差异均无统计学意义(P>0.05)。结论槲皮素对人胃癌细胞SGC-7901上皮间质转化有抑制作用,其作用机制可能是抑制AKT信号通路。
Objective To investigate the effect and mechanism of quercetin on the epithelial-mesenchymal transition of human gastric cancer cell line SGC-7901. Methods SGC-7901 gastric cancer cells were divided into control group (normal medium), LY294002 group (medium + LY294002) and quercetin group (medium + quercetin). MTT assay was used to detect the effect of quercetin on SGC- 7901, the median inhibitory concentration (IC_ (50)) of quercetin was calculated according to the cell growth inhibition rate and the value of IC_ (50) The proliferation and migration of SGC-7901 cells in three groups were detected. The protein expressions of AKT, p-AKT, Snail, Vimentin and E-cadherin in gastric cancer cells were detected by Western blot. The expression of Snail, Vimentin and E-cadherin mRNA in gastric cancer cell lines of three groups were analyzed. Results Quercetin had a significant inhibitory effect on gastric cancer cells SGC-7901 with an IC 50 value of (112.9 ± 2.05) μmol / L and a migration distance of (0.16 ± 0.03) mm in LY294002 group (0.44 ± 0.04) mm] (P <0.05). There was no significant difference between LY294002 group and quercetin group (P> 0.05) ; The expression of Snail, Vimentin and p-AKT in LY294002 group (0.760 ± 0.003,0.750 ± 0.006,0.71 ± 0.03) and quercetin group (0.750 ± 0.006,0.690 ± 0.004,0.68 ± 0.02) The expression of E-cadherin in group LY294002 (2.89 ± 0.19) and quercetin group (3.66 ± 0.18) were significantly higher than that in control group (P <0.05). In group LY294002, There was no significant difference in AKT protein expression between the two groups (1.03 ± 0.02, 1.02 ± 0.01, P <0.05). The expressions of Snail and Vimentin mRNA in LY294002 group (0.72 ± 0.02,0.78 ± 0.03) , Quercetin group (0.71 ± 0.01,0.79 ± 0.03) were significantly lower than the control group (1,1), E-cadherin mRNA expression in LY294002 group (2.51 ± 0.08), quercetin group (2.77 ± 0.16) (1) (P <0.05). In the LY294002 group, Snail, Vimentin, E-ca The expression of dherin mRNA and Snail, Vimentin, E-cadherin and p-AKT were not significantly different from quercetin group (P> 0.05). Conclusion Quercetin inhibits the epithelial-mesenchymal transition of human gastric cancer cell line SGC-7901, and the possible mechanism is that quercetin may inhibit the AKT signaling pathway.