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目的:建立高效液相色谱法测定布洛伪麻缓释片释放量的方法。方法:色谱柱为迪马C18(250 mm×4.6 mm,5μm),流动相为甲醇-三乙胺-0.04 mol·L-1磷酸二氢钠溶液(60∶0.02∶40),流速为1.0 ml·min-1,检测波长为215 nm,柱温为35℃,进样量为20μl。结果:布洛芬的线性范围为40~2 000μg·ml-1(r=1.000 0),平均加样回收率为99.2%,RSD=0.8%(n=9),最低定量浓度为0.04μg·ml-1;盐酸伪麻黄碱的线性范围为6~300μg·ml-1(r=1.000 0),平均加样回收率为98.5%,RSD=0.6%(n=9),最低定量浓度为0.06μg·ml-1。结论:该方法简便快速、准确灵敏,可用于布洛伪麻缓释片释放量的测定。
Objective: To establish a method for the determination of the release of bupivacaine sustained-release tablets by high performance liquid chromatography. METHODS: The mobile phase was methanol-triethylamine-0.04 mol·L-1 sodium phosphate monobasic (60:0.02:40) at a flow rate of 1.0 ml with a Dima C18 column (250 mm × 4.6 mm, 5 μm) Min-1, the detection wavelength was 215 nm, the column temperature was 35 ℃ and the injection volume was 20μl. Results: The linear range of ibuprofen was 40-2 000μg · ml-1 (r = 1.000 0), the average recovery was 99.2%, the RSD was 0.8% (n = 9) and the lowest concentration was 0.04μg · The linear range of pseudoephedrine hydrochloride was 6 ~ 300μg · ml-1 (r = 1.000 0) with the average recovery of 98.5%, RSD = 0.6% (n = 9) and the lowest concentration was 0.06μg · ml-1. Conclusion: The method is simple and rapid, accurate and sensitive, and can be used to determine the release of bupivacaine sustained-release tablets.