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实验方法实验动物:Wistar 系雄性大白鼠(7周龄,体重180～220g),用固体饲料单独饲养1周后,在采取胆汁前24小时断食,但取胆汁前可自由摄取饮料水。试剂:去氢胆酸钠(DHC),左旋利胆醇(1-pp),右旋-1.8-萜二烯(d-LITM),4-甲繖形酮(4-MU),去氧胆酸钠(DCA),熊去氧胆酸(UDCA),邻-羟苯水杨酸胺(p-HSA),胆酸钠(CA)。生药:槭树皮及银杏叶在当年内采集,其他则为市售商品。但其中白术为南朝鲜产品,黄芩用尖芩,均制成粉末,取30g 用甲醇300ml 温浸3次,每次2小时,将抽滤液减压浓缩成干浸膏。试药配制及用法:将 DHC、DCA、CA 用蒸馏水,UDCA 用0.4MNaOH 分别溶懈,1-PP、d-LTM、4-
EXPERIMENTAL METHODS Experimental animals: Wistar male rats (7 weeks old, weighing 180-220 g) were fed with solid feed alone for 1 week, and fed 24 hours before taking bile. However, drinking water was freely taken before taking bile. Reagents: Sodium dehydro cholate (DHC), levocabithiol (1-pp), d-LITM, 4-MU, Sodium DCA, UDCA, p-HSA and CA. Herbal Medicine: Maple bark and ginkgo biloba were collected during the year while others were marketed. However, Atractylodes macrocephala is a product of South Korea and Scutellaria baicalensis are both made into powder, 30 g of 300 ml of methanol is soaked in warm water for 3 hours for 2 hours each, and the filtrate is concentrated to a dry extract under reduced pressure. Preparation and usage of the reagent: DHC, DCA, CA with distilled water, UDCA were dissolved with 0.4M NaOH, 1-PP, d-LTM, 4-