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目的 研究流行性出血热 (EHF)肾组织中增殖细胞核抗原 (PCNA)和波形丝蛋白 (vimentin)的表达及意义。方法 应用多重PAP免疫组化方法 ,对 17例EHF尸检肾组织中PCNA和vimentin的表达进行观察。结果 EHF尸检肾组织PCNA的阳性率为 6 4 71% ,集合管的增殖指数显著大于远曲小管 (P <0 0 1) ,低血压休克期患者远曲小管和集合管上皮细胞vimentin呈强阳性表达 ,相同部位PCNA也呈阳性 ,PCNA的阳性强度与局部病变的程度相一致。结论EHF患者急性肾功能衰竭的早期就存在损伤肾小管上皮细胞的再生、增殖和分化 ,这些变化在EHF肾损伤的修复反应中具有重要的意义
Objective To study the expression and significance of proliferating cell nuclear antigen (PCNA) and vimentin in renal tissue of patients with epidemic hemorrhagic fever (EHF). Methods The expression of PCNA and vimentin in 17 renal tissues of EHF autopsy was observed by multiple PAP immunohistochemistry. Results The positive rate of proliferating cell nuclear antigen (PCNA) in the renal tissue from EHF autopsy was 6471%, and the proliferation index of collecting duct was significantly greater than that of distal tubule (P <0.01). The vimentin of far tubule and collecting duct epithelial cells were strongly positive Expression, the same part of the PCNA was also positive, positive PCNA intensity consistent with the extent of local lesions. Conclusion The early stage of acute renal failure in patients with EHF may damage the regeneration, proliferation and differentiation of renal tubular epithelial cells. These changes are of great significance in the repair response of EHF with renal injury