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[目的]观测补脾益气中药对脾气虚痴呆大鼠水迷宫实验、血清白介素1、白介素6影响。[方法]使用随机平行对照方法,将32只SPF级SD大鼠按随机数字表分为4组,空白组,痴呆模型组、脾气虚痴呆组,补脾益气组,8只/组。使用饮食不节加劳倦伤脾复合因素方法复制脾气虚模型,实验第1d,脾气虚痴呆组和补脾益气组大鼠单日游泳至耐力极限,禁食饲料,喂饲甘蓝(20~30g/只);双日喂饲猪油脂每只2mL/100g;连续15d。在此基础上使用氧化铝痴呆造模方法建立氧化铝痴呆模型,脾气虚模型造模同时,痴呆模型组、脾气虚痴呆组,补睥益气组均灌服氧化铝500mg/kg,1次/d,连续90d。灌胃干预,补脾益气组:实验16d开始,补脾益气中药(人参、白术各20g,黄芪40g,当归20g,升麻6g,柴胡、橘皮各12g,甘草6g),1.5mL/100g,连续75d;其他各组等量生理盐水。检测水迷宫学习记忆能力(定位航行时间,Morris水迷宫实验)、血清IL-1、IL-6(酶联免疫法)。[结果]实验第15d(造模15d),脾气虚痴呆组和补脾益气组大鼠表现为倦怠、懒动、蜷缩聚堆、眯眼、弓背,粪便时干时软,偶有便溏,食量与饮水量均减少,脾虚模型复制成功。实验第95d水迷宫学习记忆能力定位航行时间痴呆模型组、脾气虚痴呆组、补脾益气组均明显高于空白组(P<0.01),脾气虚痴呆组明显高于痴呆模型组(P<0.01),补脾益气组明显低于痴呆模型组和脾气虚痴呆组(P<0.01)。实验第96d血清IL-1、IL-6痴呆模型组、脾气虚痴呆组、补脾益气组均明显高于空白组(P<0.01),睥气虚痴呆组明显高于痴呆模型组(P<0.01);补脾益气组明显低于痴呆模型组和脾气虚痴呆组(P<0.01)。[结论]补脾益气中药可提升脾气虚痴呆大鼠水迷宫实验学习记忆能力(定位航行时间)、降低血清白介素1、白介素6。
[Objective] To observe the effects of Chinese herbal medicine for invigorating the spleen and invigorating the spleen on water maze test, serum interleukin 1 and interleukin 6 in spleen deficiency dementia rats. [Methods] 32 SPF SD rats were randomly divided into 4 groups, blank group, dementia model group, spleen-deficiency dementia group, Bupi Yiqi group and 8 / group by randomized parallel control method. The model of spleen-qi deficiency was duplicated by means of diet combined with fatigue and spleen injury. On day 1 of experiment, rats with spleen-deficiency dementia and Spleen-qi-enrichment group were swamped to endurance limit on one day, fasted and fed with cabbage (20-30g / Only); double feeding pig fat each 2mL / 100g; continuous 15d. On this basis, the model of Alzheimer’s disease was established by using the model of Alzheimer’s disease and the model of spleen-qi deficiency was established. At the same time, the dementia model group, the spleen-deficiency dementia group and the Buxuyiqi group were fed with 500 mg / d, continuous 90d. In the experiment of gavage and spleen qi, the rats in the spleen and replenishing qi group were started on the 16th day of experiment, and the traditional Chinese medicine of invigorating the spleen and invigorating qi (20g of astragalus and ginseng, 40g of astragalus, 20g of angelica, 6g of cohosh, 12g of Bupleurum, / 100g, continuous 75d; other groups equal amount of saline. Morris water maze test, serum IL-1, IL-6 (enzyme linked immunosorbent assay) were used to detect learning and memory abilities of water maze. [Results] The rats in the spleen deficiency dementia group and Bupi Yiqi group showed burnout, lazy movement, curled up, squinted and bowed back on the 15th day after modeling,溏, food intake and drinking water are reduced, spleen deficiency model copy success. The learning and memory abilities of water maze at the 95th day were significantly higher in dementia model group than in dementia model group (P <0.01), while those in spleen deficiency and dementia group and Bupi Yiqi group were significantly higher than those in blank group (P < 0.01). BuBu Yiqi group was significantly lower than dementia model group and spleen deficiency dementia group (P <0.01). The serum IL-1, IL-6 dementia model group, spleen Qi dementia group and Bu Pi Yi Qi group at the 96th day of experiment were significantly higher than those in the blank group (P <0.01), and the Qi deficiency dementia group was significantly higher than the dementia model group (P < 0.01). BuBu Yiqi group was significantly lower than dementia model group and spleen Qi dementia group (P <0.01). [Conclusion] Chinese herbs of tonifying the spleen and strengthening the qi can improve the learning and memory abilities of the water maze in rats with spleen deficiency dementia (positioning sailing time) and decrease the levels of serum interleukin 1 and interleukin 6.