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去泛素化酶是一类参与蛋白质稳定性调控的酶,广泛存在于真核生物细胞中,主要功能是水解底物蛋白质上的泛素链。去泛素化酶参与多种重要的生命活动,包括细胞周期调控、细胞信号转导、蛋白质降解、DNA修复及基因表达等,其异常表达与多种疾病的发生密切相关。该文以果蝇为研究对象,探讨去泛素化酶nonstop(not)对果蝇眼睛锥细胞发育分化的调控作用。结果表明,在not功能缺失的果蝇三龄幼虫眼睛成虫盘中,Cut蛋白质水平发生了显著下调;同时,在果蝇蛹期眼睛的分析结果显示,not功能缺失可导致锥细胞和初级色素细胞的分化异常。进一步研究发现,not功能缺失还可导致R1/R6光感受器细胞分化异常及转录因子D-Pax2表达水平异常。然而,not功能缺失并不影响转录因子Lozenge(Lz)的蛋白质水平及EGFR(epidermal growth factor receptor)和Notch信号通路的活性。综上所述,该文结果表明,not可能通过调控D-Pax2的表达水平与R1/R6光感受器细胞的分化影响锥细胞和初级色素细胞的分化。
Deubiquitinase is a type of enzyme that is involved in the regulation of protein stability. It is widely found in eukaryotic cells and its main function is to hydrolyse the ubiquitin chains on the substrate proteins. Deubiquitination enzymes are involved in a variety of important life activities, including cell cycle regulation, cell signaling, protein degradation, DNA repair and gene expression, and their abnormal expression is closely related to the occurrence of various diseases. In this paper, Drosophila melanogaster was used as a research object to investigate the regulatory effect of de-ubiquitination enzyme nonstop on the development and differentiation of Drosophila eye cones. The results showed that there was a significant down-regulation of Cut protein levels in adult discs of third-instar larvae with missing function, and the results of eye analysis in the pupal stage of Drosophila melanogaster indicated that the lack of function of not-induced cone cells and primary pigment cells The abnormal differentiation. Further study found that not function loss can also lead to abnormal R1 / R6 photoreceptor cell differentiation and abnormal expression of the transcription factor D-Pax2. However, the lack of function did not affect the protein level of the transcription factor Lozenge (Lz) and the activity of the epidermal growth factor receptor (EGFR) and the Notch signaling pathway. Taken together, the results of this paper suggest that not may affect the differentiation of cone cells and primary pigment cells by regulating the expression of D-Pax2 and the differentiation of R1 / R6 photoreceptor cells.