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作者用4只家兔进行实验研究,于家兔的一只眼上用划痕的方法接种Ⅰ型单疱病毒,另一只眼作为对照。于两眼上每周取材一次共3个月,制成标本,用相差显微镜和偏光显微镜检查研究,后再应用组织化学方法。单疱病毒接种后3天,可以观察到兔眼角膜表面上皮细胞DNA和RNA的量增加,组织化学染色可证明酸性粘多糖增加以及网硬蛋白(reticulin)的破坏。在早期阶段,偶而可以见到多核巨细胞。从感染后第一周开始,越来越多的细胞变成圆形和皱缩。该细胞胞浆内含有DNA弥散在RNA间,这种DNA可能是病毒。细胞核在形态、大小以及染色深度方面是不同的,在胞浆内常常可见到核的碎片。在圆形细胞中,酸性粘多
The authors used four rabbits for experimental studies, scratching herpes simplex virus type I in one eye of rabbits, and the other as a control. Draw on the two eyes once a week for a total of 3 months, made of specimens, with phase contrast microscopy and polarized light microscopy studies, and then the application of histochemical methods. Three days after inoculation of the herpes simplex virus, the amount of DNA and RNA in the epithelial cells of the cornea of rabbits was observed to be increased. Histochemical staining confirmed the increase of acidic mucopolysaccharides and the destruction of reticulin. In the early stages, you can occasionally see multinucleated giant cells. From the first week after infection, more and more cells become round and wrinkled. The cytoplasm contains DNA dispersed in the RNA, the DNA may be a virus. The nucleus is different in shape, size, and depth of staining, and nuclear fragments are often found in the cytoplasm. In round cells, acid sticky