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目的评价新型抗炎药物盐酸氯苯哌酮对神经胶质细胞炎性反应的抑制作用及机制。方法 Griess法检测一氧化氮(NO)释放、Boyden小室测定巨噬细胞趋化、ELISA法测定白细胞介素(IL)-6及RANTES分泌水平。RT-PCR法检测IL-6及RANTES基因表达,Confocal检测胞内游离Ca~(2+)水平,四甲基偶氮唑蓝(MTT)法检测细胞活力。结果盐酸氯苯哌酮可有效抑制NO释放及巨噬细胞趋化,显著抑制IL-6及RANTES分泌及基因表达,并降低胶质细胞胞内游离Ca~(2+)水平上升。结论盐酸氯苯哌酮对炎性介质介导的神经胶质细胞活化、巨噬细胞趋化及胶质细胞钙离子升高具有显著抑制作用。其中,盐酸氯苯哌酮对星形胶质细胞RANTES表达、分泌和对胶质瘤细胞内游离Ca~(2+)水平的调控作用尚属首次报道。
Objective To evaluate the inhibitory effect of a new anti-inflammatory drug, chloraphenone hydrochloride, on glioma inflammatory response and its mechanism. Methods The release of nitric oxide (NO) was measured by Griess method. The chemotaxis of macrophages was measured in Boyden chamber and the levels of interleukin (IL) -6 and RANTES were measured by ELISA. The expression of IL-6 and RANTES gene was detected by RT-PCR. The intracellular free Ca 2+ level was detected by Confocal and the cell viability was detected by MTT assay. Results Criphenone hydrochloride could effectively inhibit the release of NO and the chemotaxis of macrophages, and significantly inhibited the secretion and gene expression of IL-6, RANTES and the increase of intracellular free Ca 2+ in glial cells. Conclusion Chlobenmphenicol hydrochloride has a significant inhibitory effect on the activation of glial cells, chemotaxis of macrophages and the increase of calcium in glial cells induced by inflammatory mediators. Among them, clindamycin hydrochloride is the first report on the expression and secretion of RANTES in astrocytes and the regulation of free Ca 2+ in glioma cells.