[目的]通过Northern印迹杂交(Northern blot)验证人参中微小核糖核酸(miRNA)的存在。[方法]根据已知人参miRNA序列设计合成2条特异LNA反义寡核苷酸探针,并用地高辛标记。采用通用植物miRNA提取试剂盒提取新鲜人参Total RNA,15%变性PAGE电泳分离小RNA并检测。[结果]Northern blot表明,人参miR482和miR1439等2种miRNA均有表达。[结论]验证了人参中miRNA的存在,为研究人参的疗效机制、临床应用和剂型开发等提供新思路。 [Objective] To verify the existence of miRNA in ginseng by Northern blot. [Method] Two specific LNA antisense oligonucleotide probes were designed and synthesized based on the known miRNA sequence of ginseng and labeled with digoxigenin. The universal ginseng miRNA extraction kit was used to extract fresh ginseng total RNA, and 15% of the denatured PAGE electrophoresis was used to isolate and detect the small RNA. [Result] Northern blot showed that miRNAs of miR482 and miR1439 were all expressed in two kinds of miRNAs. [Conclusion] The existence of miRNA in ginseng was validated, which provided new ideas for studying the therapeutic mechanism, clinical application and formulation development of ginseng.