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为了探讨种子引发处理对甜高粱种子萌发和早期幼苗阶段的影响机制,分别以KCl、CaCl2溶液(50、100和200 mmol·L-1)和具有相同水势的PEG溶液为引发试剂,对种子进行吸水-回干处理,分析了“M-81E”甜高粱品种在引发处理过程中的吸湿、回干情况,引发处理后分析了种子在不同浓度NaCl溶液中发芽率、形态响应、生物量分配以及幼苗体内Na+、K+、Ca2+积累情况。结果表明:在引发处理过程中,甜高粱种子存在快速吸水期和快速失水期,分别发生在吸湿、回干的前3 h;在随后的发芽试验过程中M-81E甜高粱表现出较高的耐盐性,NaCl浓度低于200 mmol·L-1时,种子发芽率、发芽速度差异不显著,除CaCl2引发处理对50和200 mmol·L-1NaCl胁迫下种子发芽速度有显著的促进作用外(P<0.05),不同引发试剂对种子发芽率、发芽速度影响差异不显著;引发处理对幼苗胚根长、胚轴长及全长影响差异显著(P<0.05);在无盐胁迫条件下,引发处理对幼苗生长产生负效应,随着盐胁迫浓度增加,引发处理能够明显促进甜高粱幼苗生长,且不同引发处理效果具有一定的差异性;随着盐浓度升高,幼苗体内Na+、Ca2+含量显著增加,但对K+含量无显著影响;引发处理显著降低了K+含量(KCl引发除外);总体而言,不同引发方式均不同程度地增加了甜高粱幼苗生长、生物量积累以及盐胁迫条件下的离子调节能力,其中CaCl2引发效果略优于其他两种引发方式。
In order to investigate the mechanism of seed initiation on seed germination and early seedling stage of sweet sorghum, seeds were treated with KCl, CaCl2 (50, 100 and 200 mmol·L-1) and PEG solution with the same water potential Water absorption-back-drying treatment, the sorption and desiccation of sweet sorghum varieties of “M-81E” were analyzed. After the initiation treatment, the germination rate, morphological response and biomass of seed in different concentrations of NaCl solution were analyzed. Distribution and seedlings Na +, K +, Ca2 accumulation. The results showed that sweet sorghum seeds had a rapid water absorption period and a rapid water loss period, respectively, during the first 3 h of hygroscopic and dry recovery during the initiation treatment, and higher in the M-81E sweet sorghum during the subsequent germination test The salt tolerance of seed germination rate and germination rate was not significantly different when NaCl concentration was lower than 200 mmol·L-1, except that CaCl2 treatment significantly promoted seed germination rate under 50 and 200 mmol·L-1 NaCl stress (P <0.05). There was no significant difference in the germination rate and germination rate between the two treatments (P <0.05). The effect of priming treatment on radicle length, , The initiation treatment had a negative effect on seedling growth. With the increase of salt stress concentration, the initiation treatment could significantly promote the growth of sweet sorghum seedlings, and the effect of different initiation treatments was somewhat different. With the increase of salt concentration, Na + Ca2 + content significantly increased, but K + content had no significant effect; K treatment significantly reduced the K + content (except for KCl); in general, the different ways of initiation increased sweet sorghum seedling growth, biomass accumulation and salt Under forcing conditions ion regulation, which is slightly better than the effect of CaCl2 other two initiator initiator ways.