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目的评价古洛糖酸内酯在气相色谱-质谱系统中对甲拌磷、二嗪农、乐果、甲基对硫磷、马拉硫磷5种有机磷类农药检测的改善作用。方法通过比较用不同浓度(0、0.3、0.6、0.9和1.2mg/ml)古洛糖酸内酯-甲醇溶液配置的相同浓度(500ng/ml)有机磷农药峰面积的变化,确定古洛糖酸内酯的最佳用量。结果当古洛糖酸内酯溶液浓度<1mg/ml时,5种有机磷农药在相同检测条件下的峰面积随其浓度的增加呈上升趋势;当浓度等于1mg/ml时,峰面积达到最高水平;随其浓度的增加,峰面积无明显变化,且5种化合物变化趋势相同。二嗪农灵敏度提高4倍,甲拌磷灵敏度提高5倍,乐果、甲基对硫磷和马拉硫磷的灵敏度提高10倍,且峰型改善明显,拖尾现象消失。结论古洛糖酸内酯可以有效提高5种有机磷类农药检测的灵敏度,改善因活性位点引起的峰型拖尾、不对称现象,能够有效补偿基质效应引起的定量偏倚。浓度为1mg/ml时,具有最佳的补偿效应。
Objective To evaluate the effects of gulonic lactone on the detection of five organophosphorus pesticides including phorate, diazinon, dimethoate, methyl parathion and malathion in gas chromatography-mass spectrometry system. Methods Gulose was determined by comparing the change in peak area of organophosphorus pesticides with the same concentration (500 ng / ml) configured with different concentrations of gulonolactone-methanol solution (0, 0.3, 0.6, 0.9 and 1.2 mg / The best amount of lactone. Results When the concentration of gulonolactone solution was less than 1 mg / ml, the peak areas of five kinds of organophosphorus pesticides under the same test conditions increased with the increase of their concentration. When the concentration was equal to 1 mg / ml, the peak area reached the highest With the increase of its concentration, there was no obvious change of the peak area, and the trend of the five compounds was the same. The sensitivity of diazinon increased by 4 times and the sensitivity of phorate by 5 times. The sensitivity of dimethoate, methyl parathion and malathion increased by 10 times, and the peak shape was obviously improved and the trailing phenomenon disappeared. Conclusion Gulonolactone can effectively increase the sensitivity of five organophosphorus pesticides and improve peak-tailing and asymmetry caused by active sites, which can effectively compensate for the quantitative bias caused by matrix effects. At a concentration of 1 mg / ml, it has the best compensation effect.