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目的:探讨人趋化素样因子1(chemokine-like factor1,CKLF1)基因转移对急性心肌梗死大鼠梗死面积及心功能的影响。方法:雄性3月龄Sprague-Dawley(SD)大鼠30只,随机分为3组,盐水组、CKLF1组、空质粒组,分别于股直肌内注射生理盐水100μg、CKLF1真核表达质粒或空载质粒100μg,并予以直流方波电脉冲刺激,第6天结扎冠状动脉前降支构建大鼠急性心肌梗死模型,第22天行超声心动图及血流动力学检查,然后处死大鼠,测定心肌梗死面积。结果:CKLF1组,左心室射血分数(67.02%±12.24%)明显高于盐水组(43.64%±7.82%)及空质粒组(47.56%±4.10%),P<0.05;左心室短轴缩短率(33.83%±10.15%)明显高于盐水组(18.49%±3.96%)及空质粒组(20.85%±2.24%),P<0.05;左心室压力上升最大速率[(5720.01±826.32)mmHg/s,1mmHg=0.133kPa]明显高于盐水组[(3955.69±685.91)mmHg/s]及空质粒组[(4412.03±500.74)mmHg/s],P<0.05;左心室压力下降最大速率[(4636.23±407.17)mmHg/s]明显高于盐水组[(2984.82±615.24)mmHg/s]及空质粒组[(2963.87±419.36)mmHg/s],P<0.05;而CKLF1组大鼠心肌梗死面积(29.63%±3.93%)明显小于盐水组(38.01%±5.48%)及空质粒组(37.50%±6.33%),P<0.05。结论:CKLF1基因转移可以缩小大鼠心肌梗死面积并改善其心功能。
Objective: To investigate the effect of chemokine-like factor 1 (CKLF1) gene transfer on infarct size and cardiac function in acute myocardial infarction (AMI) rats. Methods: Thirty male Sprague-Dawley (SD) rats of 3 months old were randomly divided into 3 groups: saline group, CKLF1 group and empty plasmid group. Normal saline (100 μg), CKLF1 eukaryotic expression plasmid The recombinant plasmid was injected into the left anterior descending artery (LAD) on the 6th day to establish the model of acute myocardial infarction. The rats were sacrificed on the 22nd day by echocardiography and hemodynamics. Determine myocardial infarct size. Results: The left ventricular ejection fraction (67.02% ± 12.24%) in CKLF1 group was significantly higher than that in saline group (43.64% ± 7.82%) and empty plasmid group (47.56% ± 4.10% (33.83% ± 10.15%) was significantly higher than that in saline group (18.49% ± 3.96%) and empty plasmid group (20.85% ± 2.24%), P <0.05; the maximum rate of left ventricular pressure rise was (5720.01 ± 826.32) mmHg / s, 1mmHg = 0.133kPa] was significantly higher than that of saline group [(3955.69 ± 685.91) mmHg / s] and empty plasmid group [(4412.03 ± 500.74) mmHg / s], P < ± 407.17) mmHg / s] was significantly higher than that in saline group [(2984.82 ± 615.24) mmHg / s] and empty plasmid group [(2963.87 ± 419.36) mmHg / s], P < 29.63% ± 3.93%) was significantly lower than that of saline group (38.01% ± 5.48%) and empty plasmid group (37.50% ± 6.33%), P <0.05. Conclusion: CKLF1 gene transfer can reduce myocardial infarct size and improve cardiac function in rats.