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为验证哈茨木霉菌与啶酰菌胺联用对番茄灰霉病菌的增效作用,采用显微镜观察法观察了菌药联用后哈茨木霉菌和番茄灰霉病菌的菌丝形态,用菌丝生长速率法和圆盘滤膜法测定了菌药联用后哈茨木霉菌挥发性物质、非挥发性物质和粗酶液对番茄灰霉病菌的抑菌活性。结果显示:啶酰菌胺对哈茨木霉菌的菌丝基本无影响,但能使灰霉病菌的菌丝发生间断性溶解;菌药联用后哈茨木霉菌挥发性物质对灰霉病菌的最高抑菌活性为61.17%,而哈茨木霉菌和啶酰菌胺单独对灰霉病菌的抑菌活性分别为48.79%、0.97%,表现增效作用;菌药联用后哈茨木霉菌非挥发性物质和粗酶液对番茄灰霉病菌的抑菌活性未明显增强。研究表明,啶酰菌胺与哈茨木霉菌联用的增效机制是啶酰菌胺导致灰霉病菌的菌丝部分溶解,同时增强了哈茨木霉菌挥发性物质对灰霉病菌的抑菌活性。
To verify the synergistic effect of Trichoderma harzianum and boscalid on Botrytis cinerea, the mycelial morphology of Trichoderma harzianum and Botrytis cinerea were observed by microscopy, The antibacterial activities of volatile compounds, nonvolatile substances and crude enzyme solutions of Botrytis cinerea against Botrytis cinerea were determined by the rate method and disk filter method. The results showed that boscalid had no effect on the mycelia of Trichoderma harzianum, but could cause intermittent dissolution of the mycelium of Botrytis cinerea. The highest inhibition of Botrytis cinerea by volatile compounds of Trichoderma harzianum The antibacterial activity of Trichoderma harzianum and Trichoderma harzianum alone was 61.17%, while that of Trichoderma harzianum and Boscalid alone was 48.79% and 0.97%, respectively. The antimicrobial activity of crude enzyme solution against Botrytis cinerea did not significantly increase. The research shows that the synergistic mechanism of boscalid and Trichoderma harzianum is that boscalid causes the partial mycelium dissolution of Botrytis cinerea, and enhances the bacteriostasis activity of Trichoderma harzianum to Botrytis cinerea.