论文部分内容阅读
应用凝胶电泳和免疫印斑技术对日本血吸虫卵与卫氏并殖吸虫成虫的水溶性抗原(JSEA、PAA)和尿素溶性抗原(JEA-u、PAA-u)进行了抗原蛋白组分比较分析。结果显示:两者的特异性抗原显色主带,JSEA、JEA-u分别有8条和3条,PAA、PAA-u分别有9条和2条;其中JSEA、JEA-u分别有1条和2条,PAA、PAA-u分别有1条和2条对异种抗血清呈现交叉反应显色带。表明两种吸虫的可溶性抗原均存在共同抗原组分。慢性血吸虫病患者血清与并殖吸虫成虫抗原的交叉反应仅见于PAA,而未见于PAA-u,提示PAA-u在避免交叉反应和抗原纯化方面比PAA较为优越。
The antigenic protein components of the water soluble antigen (JSEA, PAA) and urea soluble antigen (JEA-u, PAA-u) of Schistosoma japonicum and Paragonimus westermani adults were analyzed by gel electrophoresis and immunoblotting. . The results showed that there were 8 and 3 JSEA and JEA-u, respectively, and 9 and 2 PAA and PAA-u, respectively, of which JSEA and JEA-u had 1 And 2, PAA, PAA-u, respectively, one and two anti-antiserum showed cross-reaction with the color band. The results showed that there were common antigenic components in the soluble antigens of two kinds of flukes. Chronic schistosomiasis serum and Paragonimus adult antigen cross-reaction only seen in PAA, but not in PAA-u, suggesting that PAA-u in avoiding cross-reaction and antigen purification than PAA superior.