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作者分别从小牛脾脏和猪血浆中提取了粒细胞生成抑制因子(GIF)和血浆非特异性免疫抑制蛋白、(PNIP),并对它们的生物学活性进行检测。GIF能够明显抑制BaBL/C小鼠骨髓粒-巨噬细胞集落形成单位(GM-CFU),但对小鼠脾脏细胞及小鼠淋巴瘤L5178Y细胞的~3HTdR掺入无明显作用;PNIP能够显著地抑制C57BL/6J小鼠脾脏和人外周血淋巴细胞的增殖反应,同时也能够抑制小鼠淋巴瘤L5178Y细胞和人淋巴瘤Raji细胞的生长,但对非淋巴细胞系列的Hela细胞和Vero-E6细胞则无明显作用。GIF和PNIP均可使相应靶细胞的辐射敏感性降低。经GIF处理的骨髓细胞,受照射后其GM-CFU较对照组明显增多;经PNIP处理的L578Y细胞受照射后其细胞生存率和~3H-TdR掺入率亦均较对照组为高。组织抑制因子的辐射防护作用与它们作用于靶细胞的时间和浓度以及照射剂量的大小密切相关。
The authors extracted granulocyte growth inhibitory factor (GIF) and plasma nonspecific immunosuppressive protein (PNIP) from calf spleen and porcine plasma, and tested their biological activity. GIF could significantly inhibit bone marrow granulocyte-macrophage colony forming unit (GM-CFU) in BaBL / C mice, but had no effect on ~ 3HTdR incorporation in mouse spleen cells and mouse lymphoma L5178Y cells; Inhibited the proliferation of spleen and human peripheral blood lymphocytes in C57BL / 6J mice, and also inhibited the growth of mouse lymphoma L5178Y cells and human lymphoma Raji cells. However, non-lymphocyte series of Hela cells and Vero-E6 cells No significant effect. Both GIF and PNIP can reduce the radiation sensitivity of the corresponding target cells. The GIF-treated bone marrow cells showed significantly increased GM-CFU after irradiation compared with the control group. The cell viability and ~ 3H-TdR incorporation rate of PNIP-treated L578Y cells were also higher than that of the control group. The radioprotective effects of tissue inhibitors are closely related to the time and concentration they act on the target cells and the size of the irradiated dose.