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Aim:To investigate the effects of triptolide,a diterpenoid triepoxide fromTripterygium wilfordii Hook F(TWHF),on the co-stimulatory molecule expres-sion and interleukin-12(IL-12)production from THP-1 cells.Methods:THP-1 cellswere differentiated into macrophage-like cells by Me_2SO,and then cultured withIFN-γ(500kU/L)and lipopolysaccharide(LPS)(1mg/L)with or without triptolide.The surface molecule expressions were analyzed on a FACScan flow cytometer.IL-12p40,IL-12p70 were assayed by ELISA.Results:Tripolide suppressed CD80and CD86 expressions on IFN-γ(500kU/L)and LPS(1mg/L)activated THP-1 cellsat nontoxic dosages of 2.5-0.625μg/L.Furthermore,the production of IL-12p40and IL-12p70 were also significantly reduced in THP-1 cells exposed to triptolide.Conclusion:Triptolide impairs the antigen-presenting function by inhibiting CD80and CD86 expressions and decreased IL-12p40 and IL-12p70(bioactive form)pro-ductions from the activated THP-1 cells.
Aim: To investigate the effects of triptolide, a diterpenoid triepoxide from Tripterygium wilfordii Hook F (TWHF), on the co-stimulatory molecule expres-sion and interleukin-12 (IL-12) production from THP-1 cells. Methods: THP-1 Cellswere differentiated into macrophage-like cells by Me_2SO, and then cultured withIFN-γ(500kU/L)and lipopolysaccharide(LPS)(1mg/L)with or without triptolide.The surface molecule expressions were analyzed on a FACScan flow cytometer.IL- 12p40,IL-12p70 were assayed by ELISA.Results:Tripolide suppressed CD80and CD86 expressions on IFN-γ(500kU/L) and LPS (1mg/L)activated THP-1 cellsat nontoxic dosages of 2.5-0.625μg/L.Furthermore, The production of IL-12p40and IL-12p70 were also significantly reduced in THP-1 cells exposed to triptolide.Conclusion:Triptolide impairs the antigen-presenting function by inhibiting CD80and CD86 expressions and decreased IL-12p40 and IL-12p70(bioactive form)pro -ductions from the activated THP-1 cells.