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Objective:To study the effect of VEGF antisense oligodeoxynucleotide(VEGF ASODN)on VEGF expression in acute monocyte leukemic cell line U937 in vitro.Methods:U937 cells were incubated with VEGF ASODN(final concentra-tion as follows:10,20 and 30 μmol/L respectively)or scrambled sequence,compared with negative control.The expression of VEGF mRNA was measured by semi-quantitative RT-PCR,VEGF protein was measured by Western blot.Results:VEGF ASODN obviously inhibited expression of VEGF mRNA in U937 cell,compared with scrambled sequence and negative control(P<0.05).And the inhibition effect was most remarkable after 24 h,which is related with the dose of VEGF ASODN(P<0.05).Scrambled sequence groups had no significant difference compared with negative control groups(P>0.05).VEGF ASODN obvi-ously inhibited expression of VEGF protein,compared with scrambled sequence and negative control(P<0.05).Conclusion:The expressions of VEGF at mRNA and protein levels in leukemic cell line U937 are down-regulated after being treated with VEGF ASODN.
Objective: To study the effect of VEGF antisense oligodeoxynucleotide (VEGF ASODN) on VEGF expression in acute monocytic leukemic cell line U937 in vitro. Methods: U937 cells were incubated with VEGF ASODN (final concentration-dependent as: 10,20 and 30 μmol / L respectively) or scrambled sequence, compared with negative control. The expression of VEGF mRNA was measured by semi-quantitative RT-PCR, VEGF protein was measured by Western blot.Results: VEGF ASODN obviously inhibited expression of VEGF mRNA in U937 cell, Compared with the scrambled sequence and negative control (P <0.05) .And the inhibition effect was most remarkable after 24 h, which is related with the dose of VEGF ASODN (P <0.05) .Scrambled sequence groups had no significant difference compared with negative control groups (P> 0.05) .VEGF ASODN obvi-ously inhibited expression of VEGF protein, compared with scrambled sequence and negative control (P <0.05) .Conclusion: The expressions of VEGF at mRNA and protein levels in leukemic cell line U937 are down- r egulated after being treated with VEGF ASODN.