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目的:研究氟离子植入猪骨源羟基磷灰石对破骨样细胞体外生物学行为的影响。方法:化学浸泡结合高温烧结法制备氟化猪骨源羟基磷灰石,以小鼠单核巨噬细胞株RAW264.7诱导形成破骨样细胞,扫描电镜观察破骨样细胞在材料表面黏附、分化与功能,CCK-8法检测材料上前破骨样细胞的增殖活性,酶活性定量检测细胞内抗酒石酸酸性磷酸酶(Tartrate resistant acid phosphatase,TRAP)活性。结果:破骨样细胞可在氟离子植入猪骨源羟基磷灰石上黏附、分化并发挥吸收功能,材料对前破骨样细胞的增殖活性无明显抑制作用,但可降低破骨样细胞TRAP的表达。结论:氟离子植入猪骨源羟基磷灰石可抑制破骨样细胞的骨吸收作用。
OBJECTIVE: To study the effect of fluoride ion implantation on the biological behavior of osteoclast-like cells in vitro. METHODS: Fluorinated porcine bone derived hydroxyapatite was prepared by chemical immersion combined with high temperature sintering. Osteoclast-like cells were induced by RAW264.7 in mouse monocyte-macrophage cell line. The osteoclast-like cells adhered to the surface of the material by scanning electron microscopy. Differentiation and function. The proliferation activity of the former osteoclast-like cells on the material was detected by CCK-8 method. The activity of intracellular tartrate resistant acid phosphatase (TRAP) was detected by enzyme activity assay. Results: Osteoclast-like cells could adhere to and differentiate into fluoride-implanted porcine bone hydroxyapatite and exert its absorption function. The material did not significantly inhibit the proliferation activity of pre-osteoclasts but reduced osteoclast-like cells TRAP expression. CONCLUSION: Fluoride ion implantation of porcine bone-derived hydroxyapatite can inhibit bone resorption of osteoclast-like cells.