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目的 建立一种新型全身炎症反应综合征 (SIRS)动物模型 ,并探讨SIRS与多器官功能障碍综合征(MODS)之间的关系。方法 采用大鼠盲肠结扎穿刺 (CLP)法 ,复制SIRS模型 ,分析手术前后SIRS诊断指标变化。结果 ①实验组大鼠术后体温升高、呼吸加快 ,白细胞计数进行性降低 (P <0 0 5或<0 0 1) ;②术后 2 4h ,血浆纤维蛋白原、炎症介质、补体C3、C4 、PGE2 含量明显升高 (P <0 0 5或 <0 0 1) ;③皮肤迟发型超敏反应增强 (耳肿百分率增加 )。④肺系数、总胆红素、谷丙转氨酶、肌酐含量明显增高 ,两组相比 ,差异有显著性 (P <0 0 5或 <0 0 1) ;⑤病理形态检查异常 ,显示多脏器炎性改变 ;⑥SIRS与MODS的发病率和病死率均相对集中于2 4～ 4 8h ,两者密切相关。结论 该模型为一理想的内源性感染和全身炎症反应综合征动物模型 ,对MODS发病机制的研究具有重要意义。
Objective To establish a novel animal model of systemic inflammatory response syndrome (SIRS) and investigate the relationship between SIRS and multiple organ dysfunction syndrome (MODS). Methods The cecal ligation and puncture (CLP) method was used to replicate the SIRS model and analyze the changes of SIRS before and after surgery. Results ① After operation, body temperature increased, respiration accelerated and leukocyte count decreased progressively in experimental group (P <0.05 or <0.01); ② At 24 h after operation, plasma fibrinogen, inflammatory mediators, complement C3, C4, PGE2 levels were significantly increased (P <0 05 or <0 0 1); ③ skin delayed type hypersensitivity increased (percentage of ear swelling). ④The lung coefficient, total bilirubin, alanine aminotransferase and creatinine were significantly higher in the two groups (P <0.05 or <0.01); ⑤Pathological examination was abnormal and showed multiple organs Inflammatory changes; ⑥SIRS and MODS morbidity and mortality are relatively concentrated in 24 ~ 48h, both closely related. Conclusion This model is an ideal animal model of endogenous infection and systemic inflammatory response syndrome. It is of great significance to study the pathogenesis of MODS.