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目的:探讨慢性乙型肝炎前C区终止变异的临床意义。方法:建立一种简单而灵敏度高的选择性聚合酶链反应(PCR)检测乙型肝炎病毒前C终止变异。结果:在47例慢性乙型肝炎中检出前C终止变异38例(81％),其在慢性肝炎、肝硬化、慢性重型肝炎中的检出率分别为82％(28/34)、80％(8/10)和67％(2/3),在HBeAg日性和抗HDe阳性中分别为76％(13/17)和83％(25/30),P>0.05。结论:前C终止变异在HBeAg阳性慢性肝病中亦呈高频率检出,抗HBe阳性慢性肝病并不都与前C终止变异有关。
Objective: To investigate the clinical significance of termination mutation in pre-C region of chronic hepatitis B Methods: To establish a simple and sensitive selective polymerase chain reaction (PCR) for detection of pre-C termination mutations in hepatitis B virus. Results: Among the 47 chronic hepatitis B cases, 38 cases (81%) were detected with pre-C termination mutation, and the positive rates were 82% (28/34) in chronic hepatitis, cirrhosis and chronic severe hepatitis, 80 % (8/10) and 67% (2/3), respectively, were 76% (13/17) and 83% (25/30), respectively, in HBeAg-positive and anti-HDe positive P> 0.05. Conclusion: The pre-C termination mutation is also detected at high frequency in HBeAg-positive chronic liver disease, and anti-HBe-positive chronic liver disease is not related to the pre-C termination mutation.