为了建立一种特异性旋毛虫病血清学诊断方法,进行了本项研究。以200条活的旋毛虫幼虫经口感染BALB/c小鼠,于感染后20d,腹腔内注射旋毛虫幼虫粗提抗原蛋白200μg。4d后,用ELISA测到高滴度的抗旋毛虫抗体,即取小鼠脾细胞与骨髓瘤细胞进行融合,对筛选出的杂交瘤细胞中的一株TS 3G6作进一步研究。经鉴定表明,由TS3G6产生的McAb对啮齿类、猪和人的血清 In order to establish a serological diagnosis of a specific trichinosis, this study was conducted. BALB / c mice were orally infected with 200 live Trichinella spiralis larvae and injected intraperitoneally with Trichinella spiralis larvae at 20 days after infection. After 4 days, high titers of anti-Trichinella antibodies were detected by ELISA, that is, mouse spleen cells were fused with myeloma cells, and one TS 3G6 in the selected hybridoma cells was further studied. It has been identified that McAbs produced by TS3G6 respond to rodents, swine and human sera