论文部分内容阅读
一种简单而有效的分离多种核苷酸的HPLC 方法,可适用于细胞及组织中核苷酸的分析。HPLC 条件:ODS-Hypersil(5μm)填充柱(250×5mm),Varian 5000液相色谱仪及具100μl 进样管的进样器,可调波长UV 检测器(检测波长254nm),移动相流速1ml/min。梯度洗脱系统:溶剂A 为含1%(v/v)甲醇的83.3 mM 磷酸三乙铵缓冲液(pH6.0);溶剂
A simple and effective HPLC method for the isolation of multiple nucleotides is suitable for the analysis of nucleotides in cells and tissues. HPLC conditions: ODS-Hypersil (5 μm) packed column (250 × 5 mm), Varian 5000 liquid chromatograph and injector with 100 μl injection tube, tunable UV detector (detection wavelength 254 nm), mobile phase flow 1 ml / min. Gradient elution system: Solvent A is 83.3 mM triethylammonium phosphate buffer (pH 6.0) containing 1% (v / v) methanol; the solvent