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Recombinant eel GH and yeast containing chinook salmon growth hormone (reGH and rcsGH) were incorporated into gelatin and sodium alginate (reGH GS and rcsGH GS) or polymer matrix (reGH HP55) to protect the hormone from proteolytic cleavage in the stomach. The diets containing reGH GS, rcsGH GS, reGH HP55 and free reGH or uncoated rcsGH were administered to red sea bream. Feeding of reGH GS, reGH HP55 and rcsGH GS diets resulted in significant increases in body weight and fork length over those of controls. These results strongly suggest that gelatin and sodium alginate as well as polymer matrix protected the hormone from proteolytic enzymes in the gastrointestinal tract to allow the bioactive hormone to enter the circulation and eventually stimulate fish growth.
Recombinant eel GH and yeast containing chinook salmon growth hormone (reGH and rcsGH) were incorporated into gelatin and sodium alginate (reGH GS and rcsGH GS) or polymer matrix (reGH HP55) to protect the hormone from proteolytic cleavage in the stomach. The diets containing reGH GS, rcsGH GS, reGH HP55 and free reGH or uncoated rcsGH were administered to red sea bream. Feeding of reGH GS, reGH HP55 and rcsGH GS diets resulted in significant increases in body weight and fork length over those of controls. These results strongly suggest that gelatin and sodium alginate as well as polymer matrix protected the hormone from proteolytic enzymes in the gastrointestinal tract to allow the bioactive hormone to enter the circulation and eventually stimulate fish growth.