在三分三细胞悬浮培养中,用无离子水代替重蒸馏水,培养细胞的干重增加和培养细胞中莨菪碱、东莨菪碱的含量稍有降低(分别降低1.2％和2.9％)。但无离子水可大大降低电费等成本(约降低1030元/吨培养基),且容易大量制备适应未来的工业生产。用市售白糖代替蔗糖,培养细胞的干重稍增加,两种生物碱的含量降低不大(约5.4％)。但市售白糖可大大减少用量(50％)和降低成本(88％)。三分三悬浮细胞中含有丰富的淀粉酶(平均酶活性约为4.37毫克还原糖/克鲜重/小时),所以它能利用淀粉作为唯一碳源而生长。 In a three-cell suspension culture, ionized water was used instead of double distilled water to increase the dry weight of cultured cells and to reduce the content of scopolamine and scopolamine in cultured cells (1.2% and 2.9%, respectively). However, non-ionic water can greatly reduce electricity costs (about 1030 yuan / ton medium), and easy preparation for future large-scale industrial production. Commercial white sugar instead of sucrose, cultured cells slightly increased the dry weight, the two alkaloids decreased slightly (about 5.4%). Commercially available white sugar, however, significantly reduces usage (50%) and reduces costs (88%). Three-thirds of the suspended cells are rich in amylase (average enzyme activity is about 4.37 mg reducing sugar / gf / h) so it can grow with starch as the sole carbon source.