用聚合酶链反应(PCR)扩增了IDDM患者组和对照组DNADQEB_1位点第二外显子片段,并与SSO杂交。结果表明:分析DQβ链57位为非门冬氨酸(NA)/门冬氨酸(A)杂合子时DQ-α_(52)位为精氨酸或非精氨酸带来的易感性或抵抗性,可见白种人和黄种人IDDM关联格局是一致的;HLA-DQB链57位为A时与IDDM负关联,为NA时与IDDM正关联。黄种人中NA纯合子频率极低(0.16),可能是构成黄种人IDDM发病率远比白种人低的原因之一。 The second exon of DNADQEB_1 was amplified by polymerase chain reaction (PCR) in IDDM patient group and control group, and then hybridized with SSO. The results showed that the susceptibility of DQ-α (52) to arginine or non-arginine when analyzed at position 57 of DQβ chain as non-aspartic acid (NA) / aspartic acid (A) Resistance, shows that Caucasian and yellow IDDM associated pattern is the same; HLA-DQB chain 57 A negative association with IDDM, when NA is associated with IDDM. The frequency of NA homozygotes in yellow is very low (0.16), which may be one of the reasons for the low prevalence of IDDM among yellow people.