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目的:研究X线对肺癌细胞X线修复交叉互补基因2((X-ray repair cross complementing gene2,XRCC2))与XRCC3表达水平的影响,探讨DNA同源重组修复机制在肺癌放疗过程中的作用。方法:以噻唑蓝还原法(MTT)检测X线对肺腺癌细胞株A549抑制率的影响,实时荧光定量RT-PCR技术检测X线处理肺癌细胞(人肺腺癌细胞株A549)后XRCC2和XRCC3mR-NA的表达水平。结果:肺癌细胞抑制率多数情况下随X线照射时间的延长及照射剂量的增大,细胞增殖抑制率增加,呈照射时间依赖性(P<0.05)和剂量依赖性(P<0.05),除了16Gy组与32Gy组比较无统计学意义(P=0.211)。X线照射后肺癌细胞XRCC2与XRCC3 mRNA的表达水平均先增高后降低,在照射后48h表达水平达高峰(P<0.05),且随着照射剂量的增大,XRCC2与XR-CC3 mRNA的表达水平也随之增加(P<0.05)。结论:X线照射可引起肺癌细胞XRCC2与XRCC3 mRNA表达水平的明显改变,表明DNA同源重组修复机制可能在肺癌放疗耐受中起了重要的作用。
OBJECTIVE: To investigate the effect of X-ray on the expression of XRCC3 and XRCC2 in lung cancer cells and to explore the role of DNA homologous recombination repair in the radiotherapy of lung cancer. Methods: The effect of X-ray on the inhibition rate of lung adenocarcinoma A549 cells was detected by MTT assay. The expression of XRCC2 and XRCC2 in lung cancer cells (human lung adenocarcinoma cell line A549) were detected by real-time fluorescence quantitative RT-PCR. XRCC3mR-NA expression levels. Results: The inhibitory rate of lung cancer cells in most cases with prolonged X-ray irradiation and irradiation dose, cell proliferation inhibition rate increased, the irradiation time-dependent (P <0.05) and dose-dependent (P <0.05) 16Gy group and 32Gy group had no statistical significance (P = 0.211). The expression levels of XRCC2 and XRCC3 mRNA in lung cancer cells increased firstly and then decreased after X-ray irradiation, and reached the peak at 48 hours after irradiation (P <0.05). With the increase of radiation dose, the expression of XRCC2 and XR-CC3 mRNA The level also increased (P <0.05). Conclusion: X-ray irradiation can cause significant changes in the expression of XRCC2 and XRCC3 mRNA in lung cancer cells, indicating that DNA homologous recombination repair mechanism may play an important role in radiotherapy tolerance of lung cancer.