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以籼亚种 30 37、Palghar (对光抑制敏感 )和粳亚种 0 2 42 8、0 2 9(耐光抑制 )及其籼粳亚种间杂种 F1 为材料进行试验。水稻叶片中叶黄素循环 (叶黄素组分紫黄质、环氧玉米黄质、玉米黄质的含量 V、A、Z )、Pr/Pn、非光化学猝灭 (q N)、表观光合量子效率 (AQY)、PS 光化学效率 (Fv/Fm)和 D1蛋白量有明显的日变化 ,且在不同基因型水稻间有显著差异。在午间强光下 ,与籼亚种相比 ,粳亚种有较高的 AQY、Fv/Fm 和 D1蛋白量 ,较低的 (A+Z) /(A+Z+V)、Pr/Pn 和 q N 值 ,籼粳正反交 F1 杂种的上述生理指标介于双亲值之间 ,且偏向其母本。相关分析表明 ,D1蛋白量与 Fv/Fm 或 AQY之间有极显著的正相关关系 ,与 (A+Z) /(A+Z+V)或 Pr/Pn 或 q N 之间有显著的负相关关系 ,(A+Z) /(A+Z+V)与 q N 之间有极显著的正相关关系。叶黄素循环抑制剂 DTT使午间强光下玉米黄质的含量明显降低 ,结果导致 D1蛋白更多地降解。上述证据证明 ,质基因编码的 D1蛋白水平、稳定性是水稻不同基因型间叶黄素循环和 CO2 交换特性差异的生理基础 ,叶黄素循环在保护光合机构避免强光破坏中起重要作用
The indica subspecies 30 37, Palghar (sensitive to photoinhibition) and Japonica subspecies 0 2 42 8, 02 9 (tolerant to photosynthesis) and its F1 hybrid between indica and japonica were tested. Lutein cycle (content of violaxanthin, ephedrine, zeaxanthin, V / A, Z), Pr / Pn, non-photochemical quenching (qN) The AQY, PS photochemical efficiency (Fv / Fm) and D1 protein content had obvious diurnal variation, and there were significant differences among different genotypes of rice. Under midday intense light, Japonica subspecies had higher AQY, Fv / Fm and D1 protein contents, lower (A + Z) / (A + Z + V), Pr / Pn And q N values, the above-mentioned physiological indices of F1 hybrids between indica and japonica F1 hybrids were between their parents and biased towards their maternal parents. Correlation analysis showed that the amount of D1 protein had a significant positive correlation with Fv / Fm or AQY and a significant negative correlation with (A + Z) / (A + Z + V) or Pr / Pn or qN There was a significant positive correlation between (A + Z) / (A + Z + V) and q N. Lutein cycle inhibitor DTT significantly reduced the content of zeaxanthin in midday light, resulting in more degradation of D1 protein. The above evidence proves that the D1 protein level and the stability encoded by the qualitative gene are the physiological basis for the differences in the lutein cycle and the CO2 exchange characteristics between different genotypes of rice and the lutein cycle plays an important role in protecting the photosynthetic apparatus from light damage