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为创建大白菜—结球甘蓝易位系,以大白菜—结球甘蓝3号单体异附加系(AA+C3)为试材,进行了游离小孢子培养,利用结球甘蓝C02连锁群相对于大白菜的特异InDel标记对获得的小孢子植株进行鉴定,从17个小孢子植株中筛选出1个具有结球甘蓝特异条带的植株。通过对InDel标记的加密设计,明确了该植株中外源甘蓝片段的大小为1.03 Mb。利用大白菜A基因组10个连锁群上均匀分布的100个InDel标记对其进行分子鉴定,初步确定了该植株中甘蓝染色体片段位于大白菜5号染色体上。花粉母细胞减数分裂观察结果显示,该植株染色体数为20条,为添加结球甘蓝3号染色体片段的大白菜—结球甘蓝易位系。
To create a Chinese cabbage-Brassica campestris translocation line, free microspore culture was carried out using Chinese cabbage-cabbage 3 (NC + C3) Microspore plants were identified by specific InDel marker in Chinese cabbage, and one plant with specific bands of cabbage was screened from 17 microspore plants. Through the encryption design of InDel marker, it was confirmed that the size of the exogenous Brassica oleracea fragment in this plant was 1.03 Mb. A total of 100 InDel markers were evenly distributed on 10 linkage groups of A genome of Chinese cabbage. The chromosome number of cabbage was localized on chromosome 5 of Chinese cabbage. Pollen mother cell meiosis observation results show that the number of chromosomes of the plant is 20, add cabbage 3 chromosome fragment of Chinese cabbage - cabbage translocation line.