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A new,simple,rapid microbial assay was put forward for the detection of chloramphenicol(CAP)residues in Turbot muscles using luminescent bacteria DB(Photobacterium phosphoreum)as test organ-ism.By the luminescence inhibition of DB,the concentrations of CAP were determined.CAP spiked inTurbot muscle tissues ranging from 0.5 to 100μg/kg was extracted using ethyl acetate and hexane.Themethod had a good recovery of 93.69% with a minimum detection limit of 0.65μg/kg.It can be per-formed within 30min following simple extraction.The method therefore proved to be advantageous overchromatographic procedures as it was inexpensive,quite sensitive and can be adopted for rapid screeningof CAP in Turbot tissues.
A new, simple, rapid microbial assay was put forward for the detection of chloramphenicol (CAP) residues in Turbot muscles using luminescent bacteria DB (Photobacterium phosphoreum) as test organism. Bυ the luminescence inhibition of DB, the concentrations of CAP were determined .CAP spiked inTurbot muscle tissues ranging from 0.5 to 100 μg / kg was extracted using ethyl acetate and hexane. The method had a good recovery of 93.69% with a minimum detection limit of 0.65 μg / kg. It can be per-formed within 30min following simple extraction. method. proof to be advantageous overchromatographic procedures as it was inexpensive, quite sensitive and can be beaten for rapid screening of CAP in Turbot tissues.