作者用华氏呼吸检压法测定了软体动物鱿鱼太平洋褶桑鱼(Todarodes pacificus Steenstrup)的神经节部位的有机磷毒剂水解酶活力,并进行了初步分离。结果发现鱿鱼的脑、头部神经节、星状神经节均含有较丰富的水解酶,此酶可水解DFP和梭曼两种毒剂。 依利诺斯工学院Hoski曾对此酶进行过纯化,但未提及电泳纯度的鉴定。我们用0.01 M pH7.5的磷酸缓 The authors used Fahrenheit Respiratory Septure method to determine the activity of organophosphate toxin hydrolase activity in the ganglia of Todarodes pacificus Steenstrup. The results showed that the squid brain, head ganglia, stellate ganglia contain more abundant hydrolase, the enzyme can hydrolyze DFP and soman two agents. This enzyme has been purified by Illinois Institute of Technology, but no reference is made to the identification of electrophoretic purity. We use 0.01 M phosphate buffer pH7.5