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目的探讨克癃胶囊对肾虚血瘀证大鼠前列腺增生的影响及其对前列腺增生相关蛋白、相关细胞因子的影响。方法建立前列腺增生肾虚血瘀证大鼠模型,观察空白对照组(A组),模型组(B组),非那雄胺组(C组),低、中、高剂量克癃给药组(D、E、F组)大鼠前列腺和前列腺相关因子的变化。结果①C～F组大鼠前列腺湿重、前列腺指数均显著低于B组(P<0.05)。②与B组比较,C、E、F组前列腺组织中Ki-67的积分光密度明显降低(P<0.01),D组无显著性差异(P>0.05)。③与B组比较,C、D、E、F组前列腺组织中碱性成纤维细胞生长因子的积分光密度明显降低(P<0.01);但D、E、F组前列腺组织中碱性成纤维细胞生长因子的积分光密度均高于C组(P<0.05)。④与B组比较,C、D、E、F组前列腺组织中血管内皮生长因子mRNA的表达降低(P<0.05);但D、E、F组前列腺组织中血管内皮生长因子mRNA的表达均较C组为高(P<0.05)。结论克癃胶囊可显著抑制大鼠前列腺增生,且呈剂量依赖性。可能与抑制前列腺增生相关蛋白及相关细胞因子的增高及表达密切相关,从而起到抑制前列腺增生的作用。
Objective To investigate the effects of Kelud capsule on benign prostatic hyperplasia (BPH) in rats with kidney deficiency and blood stasis syndrome and its effect on related proteins and related cytokines in prostatic hyperplasia. Methods The model of benign prostatic hyperplasia kidney deficiency and blood stasis was established in rats. The rats in blank control group (group A), model group (group B), finasteride group (group C), low, D, E, F) rats prostate and prostate related factors. Results ① The wet weight and prostate index of prostate in C ~ F group were significantly lower than those in B group (P <0.05). ② Compared with group B, the integral optical density of Ki-67 in C, E, F group was significantly lower than that in group B (P <0.01), but there was no significant difference in group D (P> 0.05). (3) Compared with group B, the integral optical density of basic fibroblast growth factor in prostatic tissue in groups C, D, E and F was significantly decreased (P <0.01); however, the basic fibroblasts in prostatic tissues in groups D, E and F The integral optical density of cell growth factor was higher than that of group C (P <0.05). ④Compared with group B, the mRNA expression of VEGF in C, D, E and F groups was significantly lower than that in group B (P <0.05); however, the expression of VEGF mRNA in group D, E and F was lower than that in group B Group C was high (P <0.05). Conclusion Keshi capsule can significantly inhibit the proliferation of benign prostatic hyperplasia in rats in a dose-dependent manner. It may be related to inhibiting the proliferation and expression of related proteins and related cytokines in benign prostatic hyperplasia, which may play a role in inhibiting the proliferation of benign prostatic hyperplasia.