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目的以实验大鼠为研究对象,寻找丙烯酰胺(acrylamide,ACR)神经损伤的生理生化基础。方法 48只Wistar大鼠随机分为对照组(腹腔注射5 ml/kg生理盐水)和染毒组(腹腔注射7.5、15和30 mg/kg ACR),每组12只,雌雄各半;步态评分评价神经行为改变;组织病理学法检测背根神经节(dorsal root ganglion,DRG)(电子显微镜)、心、肝、脾、肺、肾等(光学显微镜)的超微结构改变;神经生化法(定磷)检测大鼠背根神经节中Ca2+-ATP酶、Na+-K+-ATP酶活性的变化。结果实验结束时,30 mg/kg ACR染毒组大鼠体重较对照明显降低,步态评分较对照显著增加(P<0.05),异常体征明显;背根神经节病理改变明显,心、肝、脾、肺、肾等组织未见明显病变;丙烯酰胺各染毒组大鼠钙ATP酶(Ca2+-ATPase)、钠钾ATP酶(Na+-K+-ATPase)活性均较对照组显著降低(P<0.05)。结论背根神经节ATP酶活性的变化是丙烯酰胺神经损伤的重要生理生化基础之一。
OBJECTIVE To investigate the physiological and biochemical basis of acrylamide (ACR) nerve injury in rats. Methods 48 Wistar rats were randomly divided into control group (intraperitoneal injection of 5 ml / kg saline) and exposure group (intraperitoneal injection of 7.5, 15 and 30 mg / kg ACR) The ultrastructural changes of heart, liver, spleen, lung, kidney, etc. (light microscope) were detected by histopathological method. The neurobiochemical method (P) assay of Ca2 + -ATPase, Na + -K + -ATPase activity in rat dorsal root ganglion. Results At the end of the experiment, the weight of rats in 30 mg / kg ACR group was significantly lower than that in control group (P <0.05), and the abnormal signs were obvious. The pathological changes of DRG were obvious, There was no obvious pathological changes in the spleen, lung, kidney and other tissues. The activity of Ca2 + -ATPase and Na + -K + -ATPase of rats in acrylamide group were significantly lower than those in the control group (P < 0.05). Conclusion The change of ATPase activity in dorsal root ganglion is one of the important physiological and biochemical basis of acrylamide nerve injury.