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目的 研究氯沙坦对大鼠在体心肌缺血再灌注后细胞凋亡的影响及bcl 2和bax基因表达。方法 采用末端标记、原位杂交、免疫组织化学 3种方法分别检测细胞凋亡、基因表达产物的mRNA和蛋白质 ,通过图像分析系统测量阳性染色区域的平均吸光度对原位杂交和免疫组织化学检测物质进行量化处理。结果 细胞凋亡数目手术对照组为 (38± 9)个 /HP ,假手术组为 (0～ 1 )个 /HP ,药物治疗组为 (9± 4)个 /HP ,各组间差异有非常显著性 (P <0 0 0 1 ) ;原位杂交bcl 2手术对照组为 0 0 75± 0 0 2 0 ,假手术组为 0 0 60± 0 0 1 0 ,治疗组为 0 0 76±0 0 1 4 ,免疫组织化学bcl 2手术对照组为 0 1 37± 0 0 1 4 ,假手术组为 0 0 85± 0 0 1 9,治疗组为 0 1 2 5± 0 0 2 1 ,对照组、治疗组的升高与假手术组之间差异有显著性 (P <0 0 5) ,治疗组和对照组之间差异无显著性 (P >0 1 ) ;免疫组织化学bax手术对照组为 0 0 97± 0 0 2 2、假手术组为 0 0 62± 0 0 1 4、治疗组为 0 0 62± 0 0 1 4 ,对照组的升高与治疗组、假手术组间差异有非常显著性 (P <0 0 0 1 ) ,治疗组和假手术组之间差异无显著性 (P >0 9) ;bcl 2 /bax比值手术对照组为 1 41 3 ,假手术组为 1 376 ,治疗组为 2 0 1 6。结论 氯?
Objective To study the effects of losartan on apoptosis in rat hearts after myocardial ischemia-reperfusion and the expression of bcl-2 and bax genes. Methods The mRNA and protein expressions of apoptosis and gene expression products were detected by end-labeling, in situ hybridization and immunohistochemistry respectively. The average absorbance of positive staining area was measured by image analysis system. The results of in situ hybridization and immunohistochemistry For quantification. Results The number of apoptotic cells was (38 ± 9) / HP in the control group, (0 ~ 1) / HP in the sham operation group and (9 ± 4) / HP in the drug-treated group. (P <0.01). The in situ hybridization bcl 2 surgery group was 0 0 75 ± 0 0 2 0, the sham operation group 0 0 60 ± 0 0 1 0, the treatment group was 0 0 76 ± 0 0 1 37 in control group, 0 0 37 ± 0 0 1 4 in control group, 0 0 85 ± 0 0 1 9 in control group, 0 1 25 ± 0 0 2 1 in control group , There was a significant difference between the treatment group and the sham operation group (P <0.05), there was no significant difference between the treatment group and the control group (P> 0 1); the immunohistochemical bax operation control group was 0 97 ± 0 0 2 2 in the sham group and 0 0 62 ± 0 0 1 4 in the sham group and 0 0 62 ± 0 0 1 4 in the treatment group. There was a significant difference between the control group and the sham group There was no significant difference between the treatment group and sham operation group (P> 0 9). The ratio of bcl 2 / bax was 1 41 3 in surgical control group and 1 376 in sham operation group, The treatment group was 2 0 1 6. Conclusion Chlorine?