论文部分内容阅读
1999年 8～ 10月广东沿海网箱养殖的眼斑拟石首鱼 (Sciaenopocellatus)流行一种暴发性传染病 ,经抗菌药或杀虫剂处理均无效。为查明病因 ,取病鱼镜检 ,未发现寄生虫 ,也分离不到细菌。电镜观察 ,可见病鱼的脾脏细胞内有截面为六边形、直径约 15 0nm的病毒颗粒 ,且脾、肝、肾都有一定程度的病变 ;用 2对日本所报道的虹彩病毒特异引物进行PCR扩增 ,在病鱼的脾脏样品中扩增出 2条大小与所报道的虹彩病毒扩增产物吻合的特征带 ,故初步认定虹彩病毒是眼斑拟石首鱼暴发性传染病的主要致病原
From October to October 1999, the epidemic outbreak of Sciaenopocella tus in the cage culture in Guangdong coastal areas was not viable. To identify the etiology, take diseased fish microscopic examination, found no parasites, but also isolated from bacteria. Electron microscopy showed that diseased fish spleen cells within the hexagonal cross-section, a diameter of about 15 0nm virus particles, and the spleen, liver, kidney have a certain degree of lesions; with two pairs of Japanese iridescent virus-specific primers PCR amplification, in diseased fish spleen samples amplified two size and reported iris virus amplification products consistent with the characteristics of the band, so I initially identified iridescent catfish is the main cause of fulminant Ephedra infectious diseases pathogen