论文部分内容阅读
从中药瑞香狼毒（ＳｔｅｌｌｅｒａｃｈａｍａｅｊａｓｍｅＬ．）的甲醇提取物中分离到的二萜化合物尼地吗啉（ｇｎｉｄｉｍａｃｒｉｎ），以０．０２～０．０３ｍｇ／ｋｇ腹腔注射，可使小鼠白血病Ｐ－３８８和Ｌ－１２１０腹水型的生命延长７０％和８０％。以０．０１～０．０２ｍｇ／ｋｇ腹腔注射，可使小鼠实体瘤Ｌｅｗｉｓ肺癌、黑色素瘤Ｂ－１６和结肠癌２６的生命延长４０％、４９％和４１％。应用ＭＴＴ法和克隆形成法，观察了尼地吗啉对体外培养的人白血病Ｋ５６２和胃癌Ｋａｔｏ－Ⅲ、ＭＫＮ－２８、ＭＫＮ－４５及小鼠Ｌ－１２１０的细胞生长和克隆形成抑制作用，其ＩＣ＿５０在０．００７～０．０００１２μｇ／ｍｌ范围。这表明尼地吗啉具有较强的抗癌活性，是瑞香狼毒抗癌作用的主要成分。
The diterpenoid gnidimacrin, isolated from the methanol extract of Stellerachamaejasme L., was injected intraperitoneally at a dose of 0.02 to 0.03 mg/kg to induce murine leukemia P-388 and Life of L-1210 ascites type is extended by 70% and 80%. Intraperitoneal injections of 0.01 to 0.02 mg/kg resulted in 40%, 49% and 41% increase in the life span of mice with solid tumors including Lewis lung cancer, melanoma B-16 and colon cancer26. MTT method and cloning method were used to observe the effect of nimodrphine on cell growth and colony formation of human leukemia K562 and Kato-III, MKN-28, MKN-45 and mouse L-1210 cultured in vitro. IC_50 is in the range of 0.007 to 0.00012 μg/ml. This shows that Nimodulin has a strong anti-cancer activity and is the main component of the anti-cancer effect of Stellera chamaejasme.