论文部分内容阅读
目的 :研究长期运动训练对老年小鼠骨骼肌线粒体复合物I和复合物Ⅳ活性的影响 ,并探讨其机制。方法 :以C57BL/6J雄性小鼠跑转笼为运动方式 ,通过分光光度法和极谱氧电极法测定线粒体复合物I和复合物Ⅳ的活性。结果 :随着小鼠年龄的增长 ,骨骼肌线粒体复合物I(NADH脱氢酶)活性显著下降 ,复合物IV(细胞色素氧化酶 )活性无明显变化。经过8个月运动训练的小鼠骨骼肌线粒体复合物I及复合物IV活性明显升高 ,显著高于同龄对照鼠甚至高于5月龄鼠。结论 :运动训练在一定程度上致骨骼肌线粒体功能产生适应性变化。
Objective: To study the effect of long-term exercise training on the activity of mitochondrial complex I and complex Ⅳ in skeletal muscle of aged mice and to explore its mechanism. Methods: The C57BL / 6J male mice were run in cage mode. The activities of mitochondrial complex I and complex Ⅳ were determined by spectrophotometry and polarographic oxygen electrode method. Results: With the increase of mouse age, the activity of skeletal muscle mitochondrial complex I (NADH dehydrogenase) decreased significantly, while the activity of complex IV (cytochrome oxidase) did not change significantly. The activity of mitochondrial complex I and complex IV in mouse skeletal muscle after 8 months of training was significantly higher than that of the same age control mice and even higher than that of 5-month-old mice. Conclusion: Exercise training to a certain extent caused skeletal muscle mitochondrial function adaptive changes.