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用基因枪法将带Bar－GUS双标记基因的质粒（pAHC25）转入普通春小麦品种中─60634的幼胚盾片，并获转基因植株。在轰击的经预培养3～4天的342块幼胚盾片再经筛选再生的植株中，经PCR和Southern分析表明，外源基因已稳定整合到小麦基因组中，转化率为0．88％。此外发现，幼胚培养基中用2mg／Ldicamba代替2mg／L2，4－D，可提高愈伤组织的再生能力。同时，为了保持小麦幼胚愈伤组织的分化能力，在诱导愈伤组织阶段5mg／Lbialaphos的筛选时间以不超过1个月为宜，及早转入3mg／Lbialaphos分化及壮苗培养基筛选转基因植株。
Plasmid with Bar-GUS double marker gene (pAHC25) was transformed by gene gun into immature embryos of common spring wheat cultivar -60634 and transgenic plants were obtained. In bombardment of 342 preimplantation embryos 3 to 4 days and then screening of regenerated plants, PCR and Southern analysis showed that exogenous genes have been stably integrated into the wheat genome, the conversion was 0.88% . In addition, it was found that replacing 2 mg / L, 4-D with 2 mg / L dicamba in immature embryo medium improved callus regeneration. At the same time, in order to maintain the callus differentiation ability of wheat immature embryos, the screening time of callus induction 5mg / Lbialaphos should be less than 1 month, and the transgenic plants should be screened 3mg / Lbialaphos differentiation and strong seedling culture medium as early as possible .