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为了完整保存组织结构和细胞形态,尽可能地反映有关细胞遗传特征,Sato等报道了一种新的组织石蜡包埋方法,即丙酮固定,苯甲酸脱水石蜡包埋技术。我们经过实践应用,体会到该方法较福尔马林固定,酒精脱水石蜡包埋有明显的优点,现简介如下。 1.标本与试剂:选用新鲜涎腺肿瘤标本8例,取0.3cm×1.0cm×1.0cm的组织块各二块,并平分成二组。一组10％福尔马林固定,常规石蜡包埋切片。一组用丙酮固定、苯甲酸置换,石蜡包埋切片。固定至透明试剂:①丙酮(4℃),②苯甲酸丙酮饱和液,③二甲苯。 2.操作方法与步骤:①一组织块先以预冷之4℃丙酮固定,置于-20℃冰箱内一夜(可固定一周,其染色效果不受影响),②将固定液弃去,组织置于
In order to completely preserve the histological structure and cell morphology and reflect the cytogenetic characteristics as closely as possible, Sato et al. Reported a new tissue paraffin embedding method, namely acetone fixation and benzoate dehydration paraffin embedding. Through practical application, we realized that the method is more formalin fixed, alcohol dehydration paraffin embedding obvious advantages, are as follows. 1. Specimens and reagents: Selection of fresh salivary gland tumor specimens in 8 cases, take 0.3cm × 1.0cm × 1.0cm of the two blocks of tissue, and divided into two groups. A group of 10% formalin fixed, conventional paraffin-embedded sections. A group of fixed with acetone, benzoic acid replacement, paraffin-embedded sections. Fixed to transparent reagent: ① acetone (4 ℃), ② saturated benzoic acid acetone, ③ xylene. 2. Operation method and steps: ① A tissue block is precooled at 4 ℃ with acetone and placed in a refrigerator at -20 ℃ overnight (can be fixed for a week, its staining effect is not affected), ② the fixed solution was discarded, the tissue Put in