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在建立TNT大鼠白内障的基础上,用HPLC分析了晶状体内TNT及其代谢产物,并用ESR及NBT方法检测了TNT在晶状体内的代谢过程所产生的自由基。结果表明,慢性染毒24个月的大鼠白内障晶状体内含有TNT原形和4氨基2,6二硝基甲苯代谢产物,以及在体外与正常晶状体微粒体孵育可产生TNT硝基阴离子自由基和超氧阴离子自由基。上述结果提示,TNT可进入晶状体内,在其还原代谢过程中产生硝基阴离子自由基中间产物,在有氧条件下进而产生超氧阴离子自由基,这可能是TNT导致白内障的启动因素。
Based on the establishment of cataract in TNT rats, the TNT and its metabolites in the lens were analyzed by HPLC, and the free radicals produced by the metabolism of TNT in the lens were detected by ESR and NBT methods. The results showed that the TNT prototype and 4-amino-2,6-dinitrotoluene metabolites in the rat lens exposed to chronic exposure for 24 months and the generation of TNT nitro anion free radicals and super-microspheres after in vitro incubation with normal lens microsomes Oxygen anion radicals. The above results suggest that TNT can enter the lens and produce nitroanion radical intermediates during the process of its reduction and metabolism, which in turn generate superoxide anion radicals under aerobic conditions, which may be the cause of TNT-induced cataract.