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目的：合成可用于分离基因重组干扰素－α的α－ｇａｒｏｓｅ－单克隆抗体磁性微球，并建立一套磁性亲和载体分离蛋白质的方法．方法：使用物理包埋法制备磁性ａｇａｒｏｓｅ载体，使用ＣＮＢｒ法将抗体连接在磁性微球上制备磁性亲和载体．结果：通过优化合成条件，确定单抗的合成条件为ＣＮＢｒ活化用量为２００ｇ／Ｌ磁性载体，单抗的最大偶联量为１５．１ｇ／Ｌ磁性载体．使用磁性单抗载体进行一步纯化，纯化后ＩＦＮ－α的比活性为１．０ｘ１０１１ＩＵ／ｇ，电泳后使用考马斯亮兰染色其纯度为８８％，活性回收率７０％．结论：磁性亲和技术是一个简单、快速分离蛋白质的好方法．
OBJECTIVE: To synthesize α-garose-MAb microspheres which can be used to isolate gene recombinant interferon-α and to establish a set of magnetic affinity vector to separate proteins. Methods: The magnetic agarose carrier was prepared by physical embedding method and the magnetic affinity carrier was prepared by using CNBr method to attach the antibody to the magnetic microsphere. Results: The synthetic conditions of the monoclonal antibody were determined as follows: the activation amount of CNBr was 200g / L magnetic carrier and the maximum coupling amount of the monoclonal antibody was 15.1g / L magnetic carrier. After purification, the specific activity of IFN-α was 1.0 × 1011 IU / g. After electrophoresis, the purity of IFN-α was 88% with Coomassie blue staining and the activity recovery was 70%. Conclusion: Magnetic affinity is a good, simple and fast method for protein separation.