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目的探讨几种血清梅毒螺旋体检测方法的应用价值。方法应用酶联免疫吸附试验(ELISA),胶体金快速检测试验(SYP)、梅毒螺旋体明胶凝集试验(TPPA)、甲苯胺红不加热血清学试验(TRUST)分别检测一定时间段内的735份标本,其中31例为确诊感染病例。结果对735份标本进行SYP初筛后,21份显示弱阳性;对21份弱阳性标本行TPPA法检测,3份滴度低于1∶80,余均显示阴性;ELISA法检测结果与TPPA法相同。31份阳性标本用TRUST法复核,临床无症状者的20份标本多呈弱阳性,有明显临床症状者的标本均呈强阳性。结论临床可用SYP法做为初筛试验,ELISA和TPPA法做为确认诊断试验。
Objective To explore the application value of several serological detection methods of Treponema pallidum. Methods Seven hundred and seventy specimens were collected during a certain period of time by enzyme-linked immunosorbent assay (ELISA), colloidal gold rapid test (SYP), Treponema pallidum gelatin agglutination test (TPPA) and Toluidine red unheated serological test (TRUST) , Of which 31 cases of confirmed infections. Results After the SYP screening of 735 samples, 21 samples showed weakly positive results. Twenty-one weakly positive samples were detected by TPPA method with 3 titers less than 1:80, all of which showed negative results. The results of ELISA and TPPA the same. Twenty-one positive samples were reviewed by TRUST method. Most of the 20 asymptomatic samples were weakly positive, and those with obvious clinical symptoms were strongly positive. Conclusions Clinical SYP method can be used as a preliminary screening test, ELISA and TPPA method as a diagnostic test.