论文部分内容阅读
目的观察卷烟烟气对小鼠肺泡巨噬细胞(AM)的影响,探讨调节性T(Treg)细胞与AM吞噬功能的关系。方法 BALB/c小鼠经卷烟烟气暴露1、2、3个月后处死,姬姆萨染色计数支气管肺泡灌洗液(BALF)中AM数目,吞噬D大肠杆菌(E.coli)检测AM吞噬功能,流式细胞仪检测外周血及BALF中Treg细胞的比例,蛋白液体悬浮芯片技术检测BALF炎性细胞因子。结果小鼠暴露卷烟烟气1个月,BALF中AM数显著增高,AM吞噬功能和BALF中Treg细胞比例显著降低,炎性细胞因子白介素(IL)-1β、单核细胞趋化蛋白-1(MCP-1)和巨噬细胞炎症蛋白-1α(MIP-1α)含量增高,IL-10量减少;暴露2个月,AM数量及其吞噬功能和BALF中Treg细胞比例均显著降低,MCP-1及MIP-1α含量增高,IL-1β、IL-10含量减少;暴露3个月,除IL-10含量增高外,其他观察指标与对照组比较,差异无统计学意义。结论卷烟烟气对小鼠AM功能有影响,Treg细胞与AM功能关系密切,并受炎性细胞因子调节。
Objective To observe the effect of cigarette smoke on alveolar macrophages (AM) in mice and investigate the relationship between regulatory T (Treg) cells and AM phagocytosis. Methods BALB / c mice were sacrificed 1, 2, and 3 months after exposure to cigarette smoke. The number of AMs in bronchoalveolar lavage fluid (BALF) was counted by Giemsa staining and AM phagocytosis was detected by phagocytosis with E. coli Function, the ratio of Treg cells in peripheral blood and BALF were detected by flow cytometry and the inflammatory cytokines in BALF were detected by protein liquid suspension chip technique. Results After exposure to cigarette smoke for 1 month, the number of AM in BALF was significantly increased, the phagocytosis of AM and the percentage of Treg cells in BALF were significantly decreased, the levels of inflammatory cytokines interleukin-1β, monocyte chemoattractant protein-1 MCP-1, MIP-1α and IL-10 decreased. After 2 months of exposure, the numbers of AM, the phagocytic function and the percentage of Treg cells in BALF were significantly decreased And the content of MIP-1αincreased and the content of IL-1β and IL-10 decreased. Except for the increase of IL-10 after 3 months of exposure, the other indexes had no statistical significance compared with the control group. Conclusion Cigarette smoke has an effect on AM function in mice. Treg cells are closely related to AM function and regulated by inflammatory cytokines.