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目的 :观察肠缺血 -再灌注对大鼠左心功能的影响及川芎嗪注射液对大鼠心脏的保护作用并探讨其可能机制。方法 :健康雄性 SD大鼠 ,随机分为假手术对照 ( SC)组、肠缺血 -再灌注 ( IIR)组和肠缺血 -再灌注 +川芎嗪治疗 ( IIR+ LGT)组。通过生理记录仪连续观察缺血前、缺血后 1 h和再灌注后 4h内不同时间点左心功能的动态变化。检测心肌组织超氧化物岐化酶 ( SOD)、谷胱甘肽过氧化物酶 ( GSH- PX)、黄嘌呤氧化酶 ( XO)和丙二醛 ( MDA)含量。结果 :与 SC组比较 ,IIR组大鼠肠缺血后左心室内压最大变化速率 ( LV±dp/dtmax)、左心室内压力峰值 ( LVSP)下降 ,左心室舒张末期压 ( LVEDP)明显升高 ,再灌注后 ,LV± dp/dtmax和 LVSP下降更加明显 ,LVEDP更加升高 ,IIR+ TMP组上述指标的异常变化均明显减轻。三组大鼠心肌组织的 SOD、GSH- PX、XO和 MDA含量比较 ,差异均无显著性意义。结论 :大鼠肠缺血再灌注可造成左心室功能的下降 ,川芎嗪注射液对心脏功能有良好的保护作用 ,其保护机制可能与抗内毒素及强心作用有关。
Objective : To observe the effect of intestinal ischemia-reperfusion on rat left heart function and the protective effect of Chuanxiongqin injection on rat heart and to explore its possible mechanism. METHODS: Healthy male SD rats were randomly divided into sham-operated control (SC) group, intestinal ischemia-reperfusion (IIR) group, and intestinal ischemia-reperfusion + ligustrazine treatment (IIR+ LGT) group. The dynamic changes of left cardiac function at different time points before ischemia, 1 h after ischemia, and 4 h after reperfusion were continuously observed by a physiological recorder. Myocardial tissues were measured for superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), xanthine oxidase (XO), and malondialdehyde (MDA) levels. RESULTS: Compared with SC group, the maximum rate of change of left ventricular pressure (LV±dp/dtmax), left ventricular peak pressure (LVSP), and left ventricular end-diastolic pressure (LVVED) increased significantly after intestinal ischemia in IIR rats. After reperfusion, LV±dp/dtmax and LVSP decreased more significantly, and LVEDP increased even more. The abnormal changes of the above indicators in the IIR+ TMP group were significantly reduced. There were no significant differences in the SOD, GSH-PX, XO and MDA contents in the myocardial tissue of the three groups. Conclusion :Intestinal ischemia-reperfusion can cause the decline of left ventricular function. Ligustrazine injection has a good protective effect on cardiac function. Its protective mechanism may be related to anti-endotoxin and cardiotonic effects.