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目的:探讨葛根素对大鼠脊髓继发性损伤的保护作用。方法:成年雄性SD大鼠,采用改良的Allen′s打击法制成急性脊髓损伤模型后,比较1、6、12、24、48h葛根素处理组与模型组的血浆超氧化物歧化酶(SOD),丙二醛(MDA)的差异性以及Bcl-2和Bax基因蛋白表达情况。结果:葛根素能明显提高脊髓损伤大鼠血浆SOD的活性,降低血浆MDA的含量,并使大鼠脊髓Bcl-2基因表达蛋白产物增加,Bax基因表达蛋白产物减少。结论:葛根素在脊髓损伤早期可以提高SOD的活性,降低MDA的含量,并能有效上调Bcl-2表达及下调Bax表达,对脊髓的继发性损伤有保护作用。
Objective: To investigate the protective effect of puerarin on secondary spinal cord injury in rats. METHODS: Adult male Sprague-Dawley rats were treated with a modified Allen’s struck method to prepare acute spinal cord injury models. Plasma superoxide dismutase (SOD) was compared between 1,6,12,24,48h puerarin-treated and model groups. , Malondialdehyde (MDA) differences and Bcl-2 and Bax gene protein expression. RESULTS: Puerarin could significantly increase the activity of SOD in plasma and decrease the content of MDA in spinal cord injury rats, and increase the expression of Bcl-2 gene expression protein in rat spinal cord. The protein production of Bax gene was decreased. Conclusion: Puerarin can increase the activity of SOD and decrease the content of MDA in the early stage of spinal cord injury. It can effectively up-regulate the expression of Bcl-2 and down-regulate the expression of Bax, which has a protective effect on the secondary injury of spinal cord.