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分离了香石竹(Dianthus caryophyllus)两个促分裂原活化蛋白质激酶(mitogen-ac-tivated protein kinase,MAPK)基因cDNA全长,分别命名为DcMAPK1和DcMAPK2,其中DcMAPK1与拟南芥AtMAPK6高度同源。DcMAPK1在香石竹花瓣衰老过程表达增加,乙烯处理显著促进其表达,瞬时抑制DcMAPKl后切花瓶插寿命显著延长。而DcMAPK2在花瓣衰老过程中组成型表达,乙烯处理对其表达无显著影响,瞬时抑制DcMAPK2对切花瓶插寿命无显著影响。
The full-length cDNA of two mitogen-activated protein kinases (MAPKs) from Dianthus caryophyllus was isolated and named as DcMAPK1 and DcMAPK2, respectively. DcMAPK1 was highly homologous to Arabidopsis AtMAPK6. The expression of DcMAPK1 in senescenced petal of carnation increased, ethylene treatment significantly promoted the expression of DcMAPK1, and the vase insertion life was significantly prolonged after DcMAPKl was transiently inhibited. However, DcMAPK2 was constitutively expressed in the process of petal senescence, and ethylene treatment had no significant effect on the expression of DcMAPK2. The transient inhibition of DcMAPK2 had no significant effect on the vase life.