论文部分内容阅读
目的:建立测定独一味、螃蟹甲、糙苏和萝卜秦艽中5个环烯醚萜苷含量的HPLC方法。方法:色谱柱为Symmetry C18(150 mm×4.6 mm,5μm),流动相为乙腈(A)-水(B)梯度洗脱,0~5 min:6%A;5~10 min:6%~12%(A);10~40 min:12%(A);流速1.0 mL/min;柱温20℃,检测波长235 nm。结果:sesamoside、山栀苷甲酯、7,8-de-hydropenstemoside、penstemoside和8-O-乙酰山栀苷甲酯分别在50~650μg/mL(r=0.9995),50~350μg/mL(r=0.9995),40~280μg/mL(r=0.9997),10~70μg/mL(r=0.9997),40~280μg/mL(r=0.9994)范围间与峰面积线性关系良好;平均加样回收率均在96%~104%之间,RSD均小于5%(n=6)。结论:该方法简便、准确,重复性好,可用于分析独一味、螃蟹甲、糙苏和萝卜秦艽中5个环烯醚萜苷的含量。
OBJECTIVE: To establish an HPLC method for determination of 5 iridoid glycosides in Alone, Crab A, Polysporum and Radish. METHODS: Symmetry C18 column (150 mm × 4.6 mm, 5 μm) was used as the mobile phase. The mobile phase consisted of gradient elution of acetonitrile (A) and water (B) 12% (A); 10-40 min: 12% (A); the flow rate was 1.0 mL / min; the column temperature was 20 ℃ and the detection wavelength was 235 nm. Results: The inhibitory effects of sesamoside, methylgatherin, 7,8-de-hydropenstemoside, penstemoside and 8-O-methylglucoside methyl ester at 50 ~ 650μg / mL = 0.9995), 40 ~ 280μg / mL (r = 0.9997), 10 ~ 70μg / mL (r = 0.9997) and 40 ~ 280μg / mL (r = 0.9994). The average recovery was All were between 96% and 104% with RSD less than 5% (n = 6). Conclusion: The method is simple, accurate and reproducible. It can be used to analyze the contents of five iridoid glycosides in the monogamy, the crab, the cedar and the radish.