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用循环伏安法将电子媒介体硫堇电聚合在玻碳电极表面上,使其表面形成均匀的带负电的聚硫堇膜,通过静电吸附作用吸附表面带正电荷的纳米银溶胶,接着通过静电吸附带负电的葡萄糖氧化酶,最后用聚硫堇包埋电极,从而制得性能优良的葡萄糖氧化酶(GOD)生物传感器。实验发现传感器氧化峰电流与葡萄糖的浓度在1.0×10-8~5.0×10-6mol/L(r=0.9963)范围内呈良好线性关系,检出限为5.0×10-9mol/L(S/N=3)。
Cyclic voltammetry was used to electropolymerize the electronic mediator thionine on the surface of the glassy carbon electrode to form a uniform negative polysulfide membrane on the surface of the glassy carbon electrode. Electrostatic adsorption was used to adsorb the positively charged nano-silver sol on the surface, Electrostatic adsorption of negatively charged glucose oxidase, and finally with polythioninium embedded electrode, resulting in excellent performance of glucose oxidase (GOD) biosensor. The results showed that the oxidation peak current of the sensor showed a good linear relationship with the concentration of glucose in the range of 1.0 × 10-8 ~ 5.0 × 10-6mol / L (r = 0.9963) with a detection limit of 5.0 × 10-9mol / L (S / N = 3).