目的探索通过核质分离的方法制备mtDNA。方法利用TritonX 100等非离子型去污剂能溶解除核被膜以外的所有细胞膜的特点 ,通过适当离心 ,分离细胞核/细胞质来获取mtDNA。结果所得mtDNA样品纯度产率较高 ,且无核DNA污染。结论此方法简单快速 ,方法重复性好 ,值得推广应用。 Objective To explore the preparation of mtDNA by nuclear isolation method. Methods The non-ionic detergent such as TritonX 100 was used to dissolve all cell membranes except the nuclear envelope. The mtDNA was obtained by appropriate centrifugation and separation of the nuclei / cytoplasm. Results The resulting mtDNA samples had high yields and no nuclear DNA contamination. Conclusion This method is simple and rapid, the method repeatability is good, it is worth popularizing and applying.