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旨在阐明不同柱花草品种的遗传差异。利用AFLP、SSR两种分子标记技术对7个柱花草品种进行遗传多样性分析。12对AFLP引物组合共扩出966个条带,其中多态性条带有850条,平均每对引物的多态性条带为70.8条,多态性比率为87.99%。10对SSR引物共扩出26个条带,其中多态性条带有23个,多态性比率为88.46%;10对引物的观察杂合度(Ho)的范围是0~0.7143,平均为0.1857,Shannon’s遗传多样性指数(I)平均值为0.6545,平均多态性含量(PIC)为0.727。根据非加权成对平均数法(UPGMA)进行聚类分析,获得品种聚类树形图,AFLP与SSR分析的聚类图相似性显著,7个品种明显分成两类。‘矮柱花草’与‘西卡柱花草’的遗传关系最为疏远,‘奥克雷柱花草’与‘爱德华柱花草’、‘格拉姆柱花草’与‘热研5号柱花草’关系最为密切。
The aim is to elucidate the genetic differences among different stem flowers varieties. AFLP and SSR markers were used to analyze the genetic diversity of seven columnar cultivars. A total of 966 bands were amplified from 12 AFLP primer combinations, of which 850 were polymorphic bands. The average number of polymorphic bands per primer pair was 70.8 and the percentage of polymorphic bands was 87.99%. Ten pairs of SSR primers amplified 26 bands, of which 23 were polymorphic bands with a polymorphism ratio of 88.46%. The observed heterozygosity (Ho) ranged from 0 to 0.7143 for 10 pairs of primers with an average of 0.1857 , Shannon’s genetic diversity index (I) averaged 0.6545, and average polymorphism content (PIC) was 0.727. Cluster analysis based on unweighted Pairwise Average Method (UPGMA) showed that the clustering tree was similar between AFLP and SSR. The seven cultivars were obviously divided into two groups. The genetic relationship between ’short column flowers’ and ’Sika flowers’ is the most distant. The relationship between ’Oakley column’ and ’Edward column’, ’Graham column’ and ’Radiant column 5’ are most closely related .