译文简介:F.Sanger于1955年完成胰岛素全部氨基酸序列分析,1958年获诺贝尔化学奖,又于1978年发表双脱氧DNA序列测定法,1980年再次获诺贝尔化学奖。本译文节译自Sanger的回忆录(Sanger F.Sequences,sequences and sequences.Ann Rev Biochem,1988,57:1-28)中测定胰岛素序列的一部分内容。F.Sanger于1943年获得博士学位(25岁)后就到剑桥大学A.C.Chibnall教授领导的生物化学实验室工作。Chibnall的研究课题就是分析胰岛素的氨基酸组成及其化学结构。一开始,Chibnall就建议Sanger测定胰岛素的自由氨基(实际上就是N- Translation Description: F. Sanger in 1955 to complete the analysis of all amino acid sequence of insulin, Nobel Prize in Chemistry in 1958, and in 1978 published dideoxy DNA sequencing, Nobel Prize in Chemistry again in 1980. This translation section is a part of the insulin sequence assayed in Sanger’s memoirs (Sanger F. Sequences, sequences and sequences. Ann Rev Biochem, 1988, 57: 1-28). F Sanger received his Ph.D. (19) in 1943 and went to the biochemistry lab led by A.C. Chimbnall, University of Cambridge. Chibnall’s research topic is to analyze the amino acid composition of insulin and its chemical structure. From the beginning, Chibnall suggested that Sanger measure the free amino group of insulin (actually N-